Direct HPAE-PAD Analysis of Sialic Acids in Glycoprotein Hydrolyzates

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Friday, 03 February 2012 05:06

<img src="/custom/dev_images/Thermo Fisher Scientific logo.jpg" border="0" alt="Thermo Fisher Scientific" width="120" height="28" style="float: right; margin-left: 3px; margin-right: 3px;" />Thermo Fisher Scientific is pleased to announce a new method that allows sialic acids in five representative glycoproteins to be determined by acid hydrolysis release and high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD). Application Update 180: Direct Determination of Sialic Acids in Glycoprotein Hydrolyzates by HPAE-PAD demonstrates that determination of N-acetylneuraminic acid (Neu5Ac) and N-glycolylneuraminic acid (Neu5Gc) on a Thermo Scientific Dionex CarboPac PA20 Fast Sialic Acid column is specific and direct...

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