Archaeologists examining late period Mayan containers have identified nicotine traces from a codex-style flask, revealing the first physical evidence of tobacco use by ancient Mayans. The study published in Rapid Communications in Mass Spectrometry reveals the flask is marked with Mayan hieroglyphics reading, “y-otoot ’u-may” (“the home of its/his/her tobacco”), making it only the second case to confirm that the text on the exterior of a Mayan vessel corresponds to its ancient use.